肝缺血-再灌注损伤时兔脑脊液生理生化改变

Changes of Physiology Biochemistry in Cerebrospinal Fluid Induced by Liver Ischemia-reperfusion Injury

目的观察脑脊液生理生化成分变化,初步探讨肝缺血-再灌注损伤对脑的影响。方法20只健康新西兰兔随机分为对照组和肝缺血再灌注组(IR组),检测和比较脑脊液中Na+、Ca2+、SOD和MDA含量,以及血清、脑脊液和脑组织中一氧化氮(NO)代谢产物含量。结果在肝缺血再灌注后24 h,脑脊液中Ca2+和MDA显著高于对照组(P<0.05),脑脊液中SOD活性显著降低(P<0.05)。IR组血清、脑脊液和脑组织中NO2/NO3含量均明显高于对照组(P<0.05)。结论肝缺血再灌注损伤可引起脑生化的改变;NO可能参与了肝缺血-再灌注损伤对脑功能的影响。

Objective To explore the effects of liver ischemia-reperfusion injury on the changes of physiology biochemistry in cerebrospinal fluid. Methods Twenty healthy New Zealand rabbits were randomly divided into control group and liver ischemia-reperfusion injury group （IR group）. The contents of Na^＋ ,Ca^2＋ , SOD and MDA in cerebrospinal fluid and metabolite of nitrogen oxide（NO） in blood serum , cerebrospinal fluid and brain tissues were detected and analysed. Results Compared with control group, the contents of Ca^2＋ and MDA were obviously higher（P 〈 0.05）, the activity of SOD was lower （P 〈 0.05） in cerebrospinal fluid of the IR group than control at 24 h after liver ischemia - reperfusion injury ; both contents of NO^2-/NO^3- in blood serum , cerebrospinal fluid and brain tissues were higher in IR 4 group than that in control group （P 〈 0.05）. Conclusion The biochemistry of cerebrospinat fluid can be changed by liver ischemia-reperfusion injury. NO may involve in the influence of liver ischemia-reperfusion injury on cerebral function.