大孔吸附树脂富集大蓟中总黄酮类成分的工艺研究

Study on the Separation and Purification of Total Flavone from Herb Cirsii japonici by Macroporous Absorption Resin

目的筛选适合分离和纯化大蓟总黄酮的大孔吸附树脂并确立纯化工艺参数。方法以吸附及解吸率为指标考察4种型号的树脂,确定纯化大蓟黄酮的最佳树脂。并通过单因素分析考察该树脂分离、纯化大蓟总黄酮的最佳工艺条件。结果AB-8吸附率及解吸率均高于其他3种树脂。其具体工艺条件为控制上样浓度5.2 mg/ml、流速0.5 ml/min,上样体积3.5BV,洗脱剂浓度为80%,洗脱剂用量10BV。结论AB-8型树脂在所确定的工艺条件下,纯化大蓟总黄酮效果良好,总黄酮得率可达近90%。

Objective To select the suitable resin and establish the optimal conditions for separation and purification of total flavone from herb Cirsiijaponici. Methods To determine the optimal one by inspection of four types of macroporous resin. The optimal process of purification was studied by single factor analysis . Results. The static absorption capacity, the absorption ratio and the static elution ratio of AB -8 resin was higher than the others , The optimal technological conditions were found in AB -8 as follows：sample concentration was 5.2 mg/ml ,flow rate was 0.5 ml/min ,sample volume was 3.5BV ,and 80% ethanol was used as eluting agent and its dosage was 10BV. Conclusion The type of AB - 8 showed comprehensive property in the condition and the yield of the total flavones achieved nearly 90%.