麻竹叶黄酮的毒理学实验

Toxicological Test on Flavonoids from Leaves of Dendrocalamus Latiflorus

目的利用麻竹叶黄酮进行了小鼠急性毒性实验和致突变实验。方法采用急性毒性实验、小鼠精子畸形实验、小鼠骨髓嗜多染红细胞微核实验和鼠伤寒沙门氏菌/哺乳动物微粒体实验。结果小鼠经口LD50>25 g.(kg.bw)-1,腹腔注射LD50>10 g.(kg.bw)-1;竹叶黄酮各剂量组不能使鼠伤寒沙门氏菌移码突变株和碱基置换突变株发生回复突变;没有对小鼠骨髓细胞染色体的断裂效应及纺锤体毒效应;也对小鼠精子的畸形无影响。结论竹叶黄酮是一种安全的保健食品功能因子。但对人体有无毒副作用还需作临床实验。

Objective Acute toxicity experiments and mutation experiments were conducted on mice with the fiavonoids from the leaves of Dendrocalamus latiflorus. Methods By means of acute toxicity test, malformation test of mice sperm, micronucleus test of bone marrow cell and Ames test. Results LD50（ig） for mice was more than 25 g·（kg·bw）^-1 and in LD50 （ip） for mice was more than 10 g·（kg·bw）^-1. Mutagenic action of flavonoid from bamboo leaves on TA97 ,TA9s ,TA100 and TA102 ,and toxic effect on mice marrow micronucleus as well as effect on malformation of mice sperm were all negative. Conclusion The flavonoids from leaves of Dendrocalamus latiflorus was a type of secure function factor of healthy food, but whether it has toxic or negative effect on human or not it need further observation in clinical application.