氨茶碱对急进高原者抗缺氧效应的研究

Anti-hypoxia effect of aminophylline on subjects with acute high-altitude exposure

目的:探讨氨茶碱对急进高原者抗缺氧的效应。方法:总共100名青年男性入选本试验。随机分为2组:氨茶碱组50名(A组),对照组50名(C组)。所有参试者均为从四川省(海拔400米)招募的新兵。在乘飞机从四川进入拉萨(海拔3658米)前7 d,A组和C组分别开始口服氨茶碱和安慰剂。在3个时间点(服药前、服药后7d、进入高原后3 d)抽血测定血浆一氧化氮(NO)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化氢(H2O2)、乳酸(LA)和动脉血氧饱和度(SaO2)。结果:在平原地区服药前、后两组的缺氧和氧化指标比较无统计学差异。进入高原后3 d,所有参试者的血浆SOD,CAT(仅对照组),H2O2,LA水平增加(P<0.01),NO,SaO2下降(P<0.05,<0.01)。A组的SOD,CAT,H2O2,LA水平较C组低(P<0.01),而NO和SaO2水平高于C组(P<0.05,P<0.001)。结论:氨茶碱具有抗高原缺氧和抗氧化作用。

Objective： To investigate the anti-hypoxia effect of aminophylline on subjects with acute high-altitude exposure. Methods： Totally 100 young male subjects were enrolled in this study. They were randomly divided into two groups： aminophylline group （A group, 50 cases） and control group （C group, 50 cases）. They were all newly engaged army members recruited from Sichuan province （400 meters above sea level）. Subjects of A group and C group orally took aminophylline and placebo respectively 7 days before they entering Lhasa （3 658 m above sea level） by airplane. The serum levels of nitric oxide （NO）, superoxide dismutase （SOD）, catalase （CAT）, hydrogen dioxide （H2 O2）, lactic acid （LA）, and arterial oxygen saturation （SaO2） were measured at 3 time points： before drug taken, 7 days after drug taken, and 3 days after ascending to high-altitude. Results： There were no statistical differences between two groups in hypoxia and oxidation indicators before and after drug used at plain area. The serum levels of SOD, CAT （only control group）, H2O2, LA increased （P〈0.01） and NO （P〈0.05）, SaO2 （P〈0.01） decreased markedly in all subjects 3days after ascending to high-altitude. The serum levels of SOD, CAT, H2 O2 and LA in A group were much lower than those in C group （P〈0.01）. Meanwhile, the NO and SaO2 levels in A group were higher than those in C group （P〈0. 05, P〈0. 001 respectively）. Conclusion： Aminophylline has significant anti-hypoxia and anti-oxidation effects at high altitude,