柳蚕卵黄原蛋白cDNA的克隆及序列分析

Clone and Analysis of cDNA of Vitellogenin from Actias selene Hubner

经RT-PCR和RACE合成了柳蚕卵黄原蛋白(Vg)cDNA,经序列分析其长度为5701个碱基,由一个ORF组成,编码了1 774个氨基酸。氨基酸序列中有2个保守的多聚丝氨酸区域。并推测氨基酸序列中有8个天冬酰胺连接的糖基化位点(N-linked glycosylation sites)。与樟蚕、天蚕、柞蚕、野桑蚕、家蚕、樗蚕、蓖麻蚕的VgcDNA序列比对后发现有很高的同源性,分别为83.1%、81.1%、81.0%、64.7%、64.6%、79.7%、79.2%,同其它昆虫Vg cDNA序列也具有很高的同源性。根据7种蚕(家蚕、天蚕、柞蚕、蓖麻蚕、樟蚕、野桑蚕、樗蚕)VgcDNA序列建立了系统发生树。

The eDNA sequence of vitellogenin in Actias selene Hubner is obtained through RT-PCR to the total RNA and RACE amplification to 3＇-end, which contains 5 701 bases, an opening reading frame which encodes 1 774 amino acids, composed of 8 N-linked glycosylation sites ,2 polyseme regions, Homology of bases between Actias selene Hubner, Saturnia japonica（ AB190809）, Antheraea yamamai（ AB055843 ）, Antheraea pernyi（ AB049631 ）, Bombyx mandarina （ AB055845 ）, Bombyx mori（ D13160 ）, Samia cynthia pryeri （ AB190810 ）, Philosamia cynthia ricini（ ab0555844 ）. Homology identities are 83.1% ,81.1% ,81.0 % ,64.7 % ,64.6 % ,79.7 % ,79.2 % respectively. Homology identity is high compared with other insects. A phylogenetic tree was established based on the vitellogenin eDNA of seven species, including Antheraea pernyi , Antheraea yamamai , Bombyx mori , Philosamia cynthia ricini , Bombyx mandarina , Samia cynthia pryeri ,Saturnia japonica.