纤维素酶法提取知母甾体总皂苷元工艺研究

Optimization for Technological Condition to Extract Steroidal Total Sapogenin in Anemarrhenae With Cellulase Method

目的:优化知母甾体总皂苷的提取工艺。方法:以甾体菝葜皂苷元的提取率为指标,应用L9(34)正交试验设计优化了知母中甾体皂苷的纤维素酶法提取工艺。结果:影响酶法提取甾体皂苷的主次因素为:酶用量>提取温度>固液比>水解时间。纤维素酶处理能显著提高甾体皂苷的提取率,加酶后甾体菝葜皂苷元提取率的平均值为84.6%,而未加酶的结果则为58.1%。结论:酶法提取新工艺能显著提高知母甾体皂苷的提取率。

Objective： To optimize extractive technology for steroidal total sapogenin in anemarrhenae. Methods： To use extractive rate of steroidal sarsasapogenin as the target, the extract technology of cellulase method for steroidal total sapogenin in anemarrhenae was optimized with L9 （3^4） orthogonal experiment. Result： The sequence of factors to affect steroidal saponin extract as follow, dosage of enzyme 〉 extractive temperature 〉 ratio of solid and liquid 〉 hydrolytic time. The extract used cellulose could increase the extractive rate of steroidal saponin, the average value was 84.61% in extractive rate of steroidal steroidal sarsasapogenin after adding enzyme, that was 58.1% in that of no enzyme. Conclusion： The new technology extract with enzyme method can increase the extractive rate steroidal saponin in anemarrhenae obviously.