C57小鼠受照后胸腺细胞基因表达谱的变化

Profiling of differentially expressed genes induced by ionizing radiation in thymus cells of C57 mice

目的研究受1Gyγ射线照射后一个月，C57小鼠胸腺细胞细胞基因表达谱的变化。方法使用Agilent小鼠oligo基因芯片技术，观察受照射小鼠和非受照射小鼠两组间的基因差异表达。结果在所观察小鼠21319个基因中，107个基因在受照射小鼠胸腺组织中表达上调2倍以上（其中13个上调4倍以上），9个基因表达下调超过200％（其中2个下调超过400％），这些变化基因涉及细胞的一些基本代谢活动。结论变化明显的基因功能涉及胚胎发育，组织形成与维持，免疫与应激、蛋白合成、凋亡、信号转导等，上调基因的数量远多于下调基因数量，其中编码角蛋白在胚胎发育中的作用值得注意。在变化明显的上调和下调基因功能中都涉及到P13-K，也是一个值得继续关注的现象。

Objective Microarrays was used to text changes of gene expression of thymus cell in C57 mice one moth after exposed to 1 Gy γ-ray irradiation. Methods The difference of gene express between irradiated and un-irradiated mice being analysis by Agilent mouse oligo microarrays. Results In the observed 21319 known genes, 107 up-regulated at least 2-fold （13 excess 4-fold）; whereas 9 genes were down-regulated 2-fold （2 more 4-fold）. The function of theses genes are known as some basic metabolic process. Conclusions The gene changed in the experiment involved embryo development, organ formation and maintain, immunity or stress, protein compose, apoptosis, signal transduction. The number of gene is much more than that of down-regulated. It ought to be noticed that the gens which coding horny protein in embryo development, and ought also to be noticed that the gens which involve the function of P13-K.