摘要
[目的]测定白附子片中多糖含量。[方法]用水提醇沉法提取附子多糖,苯酚-硫酸分光光度法测定多糖的含量。[结果]附子多糖的含量为（5.431±0.334）%,回收率97.26%,RSD为3.78%,线性范围为5-40μg/mL。[结论]该方法快速、准确、重现性好、便于实际应用。
[ Objective ] To establish a simple and accurate method for the detetmination of polysaccharide content of Rhizoma Typhonium Gigantei. [ Methods ] Polysacchayide extracted from Rhizoma Typhonium Gigantei by water - extraction - alcohol - precipitation method were determined by phenol sulfonic acid spectrophotometry. [ Results] The contents of polysaccharide of samples was （5. 431 -0.334） % , the average recovery was 97.26 %, standard curve was liner in the concentration range 5 -40 μg/mL. [ Conclusion ] The method is simple, rapid and reliable.
出处
《大连医科大学学报》
CAS
2007年第5期453-454,共2页
Journal of Dalian Medical University
基金
辽宁省教育厅科研基金(202200953)
关键词
白附子片
多糖
含量
苯酚-硫酸分光光度法
Rhizoma Typhonium Gigantei
polysaccharide
content
spectrophotometry
