树脂包埋半薄切片染色的改进及体会

Improving and experiencing of semi-section with resin embedded

[目的]树脂包埋半薄切片染色技术的改进及在病理诊断、细胞学细微结构研究等方面的应用。[方法]应用多色和甲苯胺蓝单一染色法对树脂包埋的半薄切片进行染色。[结果]多色染色因其有对比度,接近HE染色,可用于多种组织。甲苯胺蓝单一染色虽缺乏层次,没有对比度,但对神经组织及肌肉组织,由于其组织学特点,仍不失为首选。[结论]树脂包埋半薄切片多色染色,切片清晰、鲜艳颇似HE染色效果,具有高分辨的数种光学染色的综合特征,所显示的细胞学精细结构是任何最好的石蜡切片不能比拟的。

[ Objective ] To improve the staining technic of semi - sections with resin embedded and to be applied in pathology diagnosis and fine structure study . [ Methods ] The semi - sections with resin embedded were stained using muhicolor and single staining methods. [ Results] In muhicolor staning sections there were contrast ,similar to haematoxylin and eosin staining,then It can be used in many kinds of tissues for staining. Single staining sections were absence of levels and no contract, but it was better for nerve and muscle tissues. [ Conclusions ] It was clear , fresh and like haematoxylin and eosin staining in muhicolor semi - sections with resin embedded , it had integration characters with high resolving and many kinds optics staining, the best paraffin sections were cannot compare with it in cellular fine structra observed.