氯通道ClC-1、ClC-2在人心房肌的表达及与心房颤动的关系

Chloride channels ClC-1 and ClC-2 gene expression in human atrial tissue and their relationship with atrial fibrillation

目的研究氯通道ClC-1和ClC-2基因在人心房组织的表达及与心房颤动(AF)的关系。方法将71例风湿性心瓣膜病接受换瓣手术患者分为三组,窦性心律(SR)组31例,阵发性房颤(PAF)组7例,慢性房颤(CAF)组33例,于术中获取右心耳组织,应用半定量逆转录-聚合酶链反应(RT-PCR)检测心房组织ClC-1和ClC-2的mR-NA相对含量。结果①ClC-1、ClC-2基因在人心房组织有表达。②与SR组比较,PAF组ClC-1的mRNA表达增加但无统计学意义(1.05±0.22vs1.01±0.13,P>0.05),CAF组的表达明显增加(1.25±0.18vs1.01±0.13,P<0.001),CAF组较PAF组亦明显增加(P<0.01)。ClC-1的mRNA表达水平与左房内径、AF持续时间呈正相关[(r=0.344,P=0.003)(r=0.405,P<0.001)]。③与SR组比较,PAF组ClC-2的mRNA表达无增加(1.03±0.14vs1.04±0.15,P>0.05),CAF组的表达明显增加(1.26±0.13vs1.04±0.15,P<0.001),CAF组较PAF组亦明显增加(P<0.01)。ClC-2的mRNA表达与左房内径、AF持续时间呈正相关[(r=0.441,P<0.001)(r=0.331,P=0.005)]。结论AF患者ClC-1、ClC-2的mRNA表达水平的增加可能是心房肌电重构的分子基础。

Objective To investigate gene expression of chloride channel CIC-1 and CIC-2 in human atrial tissue and their relationship with atrial fibrillation （ AF ）. Methods Right atrial appendage specimens were obtained from 33 patients with chronic AF（CAF group） , 7 with paroxysmal AF（ PAF group） and 31 matched controls in sinus rhythm （SR group） who underwent the open-heart surgery. Semi-quantitative polymerase chain reaction （RT-PCR） was used to examine the mRNA expression of CIC-1 and CIC-2 channels in atrial tissue. Results （1）There were chloride channels CIC-1 and CIC-2 gene expressing in human atrial tissue. （2）The content of CIC-1 mRNA in CAF group was significantly higher than that in SR group（ 1.25 ±0.18 vs 1. 01 ± 0. 13 ,P 〈 0. 001 ）. There was no significant difference between PAF group （1.05 ＋0.22） and SR group although the content of CIC-1 mRNA was slightly higher than that in SR group（P 〉0.05 ）. The significant difference was observed between CAF group and PAF group（P 〈0. 01 ）. CIC-1 expression at mRNA level was positively correlated with left atrial dimension and AF duration [ （ r = 0. 344, P = 0. 003 ） （ r = 0. 405, P 〈 0. 001 ） ]. （3） The content of CIC-2 mRNA in CAF group was significantly higher than that in SR group（ 1.26 ±0.13 vs 1.03 ＋0.14,P 〈 0. 001 ） , There was no significant difference between PAF group（ 1.04 ＋ 0. 15 ） and SR group （P 〉 0. 05 ）. The significant difference was observed between CAF group and PAF group（P 〈0. 01 ）. CIC-2 expression at mRNA level was positively correlated with left atrial dimension and AF duration [ （ r =0. 44 1, P 〈 0. 001 ） （ r = 0. 331, P = 0. 005 ） ]. Conclusions Increased gene expression of chloride channel CIC-1 and CIC-2 may serve as the molecular basis of atrial tissue electrical remodeling in patients with AF.