细胞密度和血清对小鼠骨髓基质细胞体外生长的影响

Effect of different cell culture densities and sera on growth of murine bone marrow stromal cells in vitro

目的:研究不同细胞接种密度、血清浓度及种类对小鼠骨髓基质细胞体外生长的影响。方法:设置不同细胞密度、不同种类血清及血清量的培养体系,观察细胞贴壁情况,细胞80%贴壁时间,用台盼蓝排斥试验检测活细胞率,MTT法检测细胞增殖情况,HE染色光镜下观察细胞形态。结果:原代全血培养的小鼠骨髓基质细胞适宜接种密度为1～4×106/ml;细胞最适生长的血清浓度为10%～20%;与胎牛血清相比马血清对小鼠骨髓基质细胞的促增殖作用更好(P<0.05);不同种类血清培养的小鼠骨髓基质细胞类型有一定差异(P<0.05)。结论:不同细胞接种密度及血清浓度和血清种类对小鼠骨髓基质细胞体外生长有一定影响。

Objective：To study the effect of different cell culture densities and sera on growth of mice bone marrow stromal cells in vitro. Methods：Bone marrow stromal cells were cultured in DMEM/F12 containing different cell densities and sera. The rate of adhered cells,cell viability by trypan blue test,proliferation of bone marrow stromal cells by MTT and cell morphological structure with light microscope were observed, Results：The cultured cells proliferated well in DMEM/F12 containing 10% - 20% sera and the densities of 1 - 4 × 10^6/ml.It prolifrated better in horse serum than fetal bovine serum（P〈0.05）.Its types were also different in newbone calf serum,fetal bovine serums and horse serum（P〈0.05）, Conclusion. Different cell densities and sera has influence on the growth of mice bone marrow stromal cells.