黄芩HPLC数字化指纹图谱研究

Digitized fingerprints of Radix Scutellariae by HPLC

目的:建立黄芩HPLC数字化指纹图谱。方法:采用反相高效液相色谱法,Century SIL C18BDS柱(200 mm×4.6mm,5μm),流动相为1%醋酸水-2%醋酸乙腈低压梯度洗脱,检测波长280 nm,柱温(30±0.15)°C,进样量5μL。以"中药色谱指纹图谱超信息特征数字化评价系统"软件进行评价。结果:以黄芩苷峰为参照物峰,确定30个共有峰,建立了黄芩HPLC数字化指纹图谱。应用色谱指纹图谱指数F等参数对不同产地黄芩HPLC指纹图谱的超信息特征进行了数字化评价。结论:所建立HPLC数字化指纹图谱具有较好的精密度和重现性,适用于黄芩药材的质量控制。

AIM： A new method for the overall quality control of Radix Scutellariae has been established by HPLC digitized fingerprint. METHODS： The chromatographic fingerprints were obtained from injecting 5 μL of the sample solution each time on a CenturySIL C18 BDS column （200 mm ×4.6 mm, 5 μm） with the gradient elution solvent system composed of 1% acetic acid water and 2% acetic acid acetonitrile. The flow rate was 1 mL/ min, the column temperature was maintained at （30 ±0.15） ℃ and the detection wavelength was set at 280 nm. The chromatographic fingerprints were evaluated by the software of the digitized evaluation system of Traditional Chinese Medicine fingerprint with the super information characteristics. RESULTS： 30 co-possessing peaks were selected as the fingerprint peaks of Radix ScuteUariae and the similarities between the chromatographic fingerprints and the herbal drugs were calculated by taking baicalin peak as the referential peak. The chromatographic fingerprints were also evaluated by the chromatographic fingerprint index （F）. CONCLUSION： This method with good precision and reproducibility can be applied to the quality control of Radix Scutellariae.