水蛭对糖尿病肾病大鼠内皮素-1水平的影响

Effects of Hirudo on level of Endothelin-1 in diabetic nephyropathy rats

目的:研究中药水蛭对糖尿病肾病(DN)大鼠ET-1水平、肾脏功能及肾脏结构的影响。方法:链脲佐菌素(STZ)诱导DN大鼠模型,随机分成模型组,水蛭(17.5 g/kg、8.75 g/kg)治疗组,卡托普利组,同时另设正常对照组,治疗6 w,测定肾功能及肾脏指数(肾重/体重),放免法检测ET-1水平,免疫组化法检测肾脏ET-1蛋白表达,并对肾组织进行光镜和电镜观察。结果:模型组大鼠ET-1水平、表达及肾脏指数明显增高,水蛭治疗6 w后,肾脏肥大明显改善,ET-1水平、蛋白表达明显降低。结论:水蛭可纠正DN大鼠早期肾脏高滤过、高灌注,并对肾脏病变有一定的保护作用,其部分机制可能是通过下调ET-1水平及表达而实现的。

AIM ： To study the effect of Hirudo on level of Endothelin-1 （ ET-1 ）, kidney structure and function ,An diabetic nephyropathy（DN） rats. METHODS： STZ-induced DN rats were randomly divided into four groups： model group, Hirudo （ 17.5 g/kg, 8.75 g/kg） treatment group, and captopril group, in addition, normal rats for control group. All rats were given by ig for 6 weeks. Kidney function and kidney index （kidney weight/body weight） were determined, the level of ET-1 was determined by Radioimmunoassay, the expression of ET-1 in the kidney tissue by immunohistochemicai, and kidney tissue was observed by lightmicroscope and transmission electron microscope. RESULTS： The level and expression of ET-1, kidney index in DN rats significantly increased. The level and expression of ET-1 markedly decreased in Hirudo treatment group, and kidney pathologic changes of DN rats in Hirudo treatment group were improved. CONCLUSION： Hirudo can ameliorate early kidney hyperdynamic abnormality in DN rats, possess protective effect on kidney of DN rats, whose mechanism may be associated partly with a down-regulation of levels and expression of ET-1.