越橘叶斑病病原菌的鉴定

Pathogen identification of leaf spot disease on blueberry

文章在辽东地区越橘叶斑病发生严重的种苗繁育圃场采集病叶标本,经分离纯化获得一株真菌,用涂抹法回接健康植株的叶片,与自然发病症状相同。根据该病原菌在光学显微镜下的形态特征以及对其ITS和-βtubulin的核酸序列分析,结果表明,确认辽东地区发生的越橘叶斑病的病原菌是柯氏帚梗柱孢霉(Cylindro-cladium colhounii Peerally)。

Collecting infected leaves from blueberry nursery located at the east of Liaoning Province where has been undergone serious leaf spot disease. Separated and purificated one strain fungi from lesions of leaf and Koch＇s postulates were fulfilled. With morphological observation, ITS and β-tubulin sequences＇ identification, we got a result as the blueberry leaf spot disease＇ pathogen was Cylindrocladium colhounii Peerally in Liaoning Province.