四君子汤对肠道菌群失调小鼠的调整作用及机制研究

STUDY ABOUT THE REGULATION OF DECOCTION OF FOUR NOBLE DRUGS ON INTESTINAL MICROBIOLOGY DYSBIOSIS AND ITS MECHANISM

目的:研究四君子汤对肠道菌群失调小鼠的调整作用及机制。方法:应用林可霉素灌胃建立小鼠肠道菌群失调模型,然后应用四君子汤水提液进行治疗,同时设正常、阳性对照及自然恢复组,于给药6d后处死小鼠,进行肠道菌群、血中乙酸含量及肠内容物pH值检测。结果:林可霉素灌胃3d后,小鼠肠道双歧杆菌、乳酸杆菌、肠杆菌显著下降,血中乙酸含量降低,肠内容物pH值升高。持续6d治疗后,上述菌量明显上升,血中乙酸含量增高且肠内容物pH值下降。结论:四君子汤能促进正常菌群生长,其机制可能与正常菌群代谢产物乙酸含量增加有关。

Objective ：To explore the regulation of Decoction of four Noble Drugs on intestinal microbe dysbiosis and its mechanism. Methods：We establish intestinal microbe dysbiosis mice by intragastric administrating lincomycin, and then treat the mice with liquid extract of Decoction of four Noble Drugs. At the same time , nomal con- trol, positive control and spontaneous recovery group were set up. The mice were sacrificed after treating 6 days and we detect intestinal microbial population, the level of acetic acid in blood and the pH of intestinal contents. Resuits ： After instragastric administrating lincomycin 3 days, the quantity of bacillus bifidus, bacillus acidi lactici and enteric bacilli decrease obviously. The level of acidum in blood increased and the pH of intetinal contents decreased. Conclusion ： Decoction of four Noble Drugs can foster normal flora, and maybe this is caused by the increasing of normal metabolic product acetic acid.