全反式维甲酸对人乳腺癌MCF-7细胞凋亡的影响

The Effect of ATRA on the Apoptosis of Human Breast Cancer MCF-7 Cell Line

目的:探讨全反式维甲酸(ATRA)对人乳腺癌MCF-7细胞凋亡的影响及作用途径。方法:在人乳腺癌细胞株MCF-7培养基中加入一定浓度ATRA和PKC-δ的专一抑制剂rottlerin(RO)并分组,通过SubG1assay by FACS、琼脂糖凝胶电泳检测基因组DNA ladder来观察ATRA对MCF-7的影响。结果:在浓度为5μM的ATRA作用下,MCF-7的凋亡率显著高于其它各组(P<0.01),并可观察到明显梯状DNA。结论:ATRA能够诱导乳腺癌MCF-7细胞凋亡,但受PKC-δ的专一抑制剂RO的抑制。

Objective To investigate the possible pathway of the effect of all - trans refinoie acid （ATRA） on the apoptosis of human breast cancer cell line （ MCF - 7）. Methods MCF - 7 cells were cultured with different concentrations of ATRA and rottlerin, （the specific inhibitor of PKC - δ）. The effect of ATRA on the apoptosis of MCF -7 cells was examined with SubG1 assay and DNA ladder formation. Results The apoptosis rate of MCF - 7 cells cultured under the concentration of 5 μM ATRA was higher than that in any other group （P 〈 0.01 ）. Apparent DNA ladder formation also observed in this group. Conclusion ATRA can induce the apoptosis of MCF - 7 cells and this effect may be inhibited by rottlerin through its inhibition on PKC - δ.