草乌甲素对Balb/c小鼠的部分免疫功能的抑制作用

Inhibitory effect of bulleyaconitine A on some immune function in Balb/c mice

目的:研究草乌甲素对Balb/c小鼠部分免疫功能的影响。方法:Balb/c小鼠随机分为空白对照组、草乌甲素3个剂量(0.08、0.16、0.32 mg·kg^(-1),肌注7d)组、氢化可的松组(阳性对照药,10mg·kg^(-1))。小鼠d7处死,检测药物对小鼠胸腺指数、脾脏指数的影响;测定小鼠T、B淋巴细胞转化功能;酶联免疫法(ELISA)测定小鼠血清总IgG水平;中性红比色法测定小鼠腹腔巨噬细胞吞噬功能;硝酸还原酶法测定巨噬细胞培养上清一氧化氮(NO)水平。结果:草乌甲素0.32 mg·kg^(-1)能抑制致裂原刺激T、B淋巴细胞增殖(P<0.01),降低腹腔巨噬细胞培养上清中NO水平(P<0.05)。草乌甲素(0.16、0.32 mg·kg^(-1))降低小鼠的胸腺指数,降低小鼠血清中总IgG水平(P<0.05,P<0.01),草乌甲素还能抑制腹腔巨噬细胞吞噬功能(P<0.05,P<0.01)。结论:草乌甲素对Balb/c小鼠的免疫功能有一定的抑制作用。

AIM： To determine the effect of bulleyaconitine A （BLA） on some immune functions of mice. METHODS： Balb/c mice were divided randomly into control group, BLA groups （0.08, 0.16, 0.32 mg. kg^-1, intramuscularly）, and hydrocortisone （10 mg.kg^-1） group, and drugs were given for 7 d. After mice were sacrificed on d 7, spleen-and thymus-index were recorded, and lymphocytes proliferation were tested. The levelof total IgG in serum was measured by ELISA method. Phagocytosis of peritoneal macrophages （MΦ） was tested with neutral-red phagocytosis assay. Nitric oxide （NO） in supernatants of macrophages was detected by nitrate reductase method. RESULTS： BLA 0.32 mg.kg^-1 inhibited T- and B-lymphocytes proliferations （P 〈 0.01 ） and NO in supernatants （P 〈 0.05） . BLA 0.16 and 0.32 mg.kg^-1 lowered the thymus-index （P 〈 0.05, P 〈 0.01 ） with the suppression of phagocytosis function of MΦ （P 〈 0.05, P 〈 0.01）. BLA also reduced the total IgG in serum （P 〈 0.05, P 〈 0.01）. CONCLUSION： BLA had the suppressive effect on some immune function of Balb/c mice.