高效液相色谱法测定盐酸罂粟碱卵磷脂微乳含量

Determination of Papaverine Hydrochloride Inlecithin Micromulsion by HPLC

目的建立测定盐酸罂粟碱-卵磷脂微乳含量的高效液相色谱法。方法采用C18柱（250mm×4．6mm，5μm）；以甲醇-水-三乙胺（60：40：0．001）（V：V：y）为流动相，流速1.0mL·min^-1，检测波长为248nm。结果盐酸罂粟碱在0.1812-0．6040mg·mL^-1浓度范围进样量与峰面积呈良好的线性关系（r=0．9999）；微乳平均回收率为99．46％，RSD为0．4％（n=5），药物保留时间为9-10min。结论该方法结果准确，重现性较好。可用于盐酸罂粟碱-卵磷脂微乳的含量测定和盾量控制。

Objective To establish a method for determining content of papaverine hydrochloride in lecithin micromulsion by HPLC. Methods C18 column(250 mm×4.6 mm,5 μm) was used,methanol-water-triethylamine(60:40:0.001,V:V:V)was used as mobile phase,and the flow rate was 1.0 mL·min-1,the detecting wave length was at 248 nm. Results There was a good linear relationship between the sample size and peak area of papaverine hydrochloride in range of 0.181 2～0.604 0 μg·mL-1(r=0.999 9).The average recovery rate was 99.46% with RSD=0.4%(n=5),the reserved time of drug was 9～10 min. Conclusion The method is highly repeatable and accurate which can be used in content dermination and quality control of papaverine hydrochloride in lecithin micromulsion.