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SOD对表没食子儿茶素没食子酸酯抗肿瘤活性及稳定性的影响 被引量：1

Effect of SOD on Anti-cancer Activity and Stability of Epigallocatechin-3-gallate
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摘要 采用MTT方法研究表没食子儿茶素没食子酸酯(EGCG)对人结肠癌细胞HT-29、人乳腺癌细胞MCF-7和人宫颈癌细胞Hela生长的影响。通过检测培养基中乳酸脱氢酶的活力研究EGCG对三种细胞的细胞毒作用,用流式细胞仪检测EGCG对HT-29的凋亡影响,用HPLC方法分析EGCG在培养基DMEM/F-12中的稳定性。以上研究均设加入SOD和不加SOD两个对照组。研究表明:与没有SOD存在条件下相比,加入SOD后,细胞存活率下降,培养基中LDH活性增强,HT-29细胞凋亡率上升;EGCG在培养基中的稳定性增强。 Effects of epigallocatechin-3-gallate （EGCG） on the growth of human colonic cancer cells HT-29, human breast cancer MCF-7 and human cervical cancer Hela were investigated by MTC assay. Cytotoxicity of EGCG on these cells was detected by determining the activity of LDH in culture medium. Induction of apoptosis in HT-29 cell after the EGCG treatment was analyzed by flow cytometry. Stability of EGCG in DMEM/F-12 was determined by HPLC and all studies above were compared in the presence or absence of SOD. Investigation showed that cells survival percentage deceased and activity of LDH in the culture medium increased after adding SOD. The apoptotic rate of HT-29 and the stability of EGCG in culture increased in the presence of SOD comparing to absence of SOD.
作者 张斌 夏艳秋 金礼吉 徐永平
机构地区 大连理工大学环境与生命学院生物科学与工程系
出处 《茶叶科学》 CAS CSCD 北大核心 2007年第4期293-298,共6页 Journal of Tea Science
基金 国家杰出青年基金(30125034)
关键词 EGCG SOD HT-29细胞 EGCC, SOD, HT-29 cells
分类号 R73-3 [医药卫生—肿瘤]
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参考文献10

  • 1Chi C,Guoxiang S,Vidya H,et al.Epigallocatechin-3-gallate-induced stress signals in HT-29 human colon adenocarcinoma cells[J].Carcinogenesis,2003,24(8):1369-1378.
  • 2Masatoshi Yokoyama,Mitsuyo Noguchi,Yoshifumi Nakao,et al.The tea polyphenol,(-)-epigallocatechin gallate effects on growth,apoptosis,and telomerase activity in cervical cell lines[J].Gynecologic Oncology,2004,92(1):197-204.
  • 3Mittal A,Pate MS,Wylie RC,et al.EGCG down-regulates telomerase in human breast carcinoma MCF-7 cells,leading to suppression of cell viability and induction of apoptosis[J].Int J Oncol.,2004,24(3):703-710.
  • 4陈勋,于海宁,沈生荣.EGCG与锌离子互作对前列腺癌细胞PC-3生长的影响[J].茶叶科学,2006,26(3):219-224. 被引量：5
  • 5Mochizuki M,Yamazaki S,Kano K,et al.Kinetic analysis and mechanistic aspects of autoxidation of catechins[J].Biochim Biophys Acta,2002,1569(1-3):35-44.
  • 6Nakayama T,Enoki Y,Hashimoto K.Hydrogen peroxide formation during catechin oxidation is inhibited by superoxide dismutase[J].Food Sci Technol Int,1995(1):65-69.
  • 7Miura YH,Tomita I,Watanabe T,et al.Fukui S.Active oxygens generation by flavonoids[J].Biol Pharm Bull,1998,21(2):93-96.
  • 8Yang CS,Maliakal P,Meng X.Inhibition of carcinogenesis by tea[J].Annu ReV Pharmacol Toxicol,2002(42):25-54.
  • 9Hong J,Lu H,Meng X,et al.Stability,cellular uptake,biotransformation,and efflux of tea polyphenol (-)-epigallocatechin-3-gallate in HT-29 human colon adenocarcinoma cells[J].Cancer Res,2002,62:7241-7246.
  • 10Zhe H,Shengmin S,Hui Y,et al.Mechanism of Action of (-)-Epigallocatechin-3-Gallate:Auto-oxidation-Dependent Inactivation of Epidermal Growth Factor Receptor and Direct Effects on Growth Inhibition in Human Esophageal Cancer KYSE 150 Cells[J].Cancer Res,2005,65 (17):8049-8056.

二级参考文献21

  • 1Rebbeck T R.Genetics,disparities,and prostate cancer[J].LDI Issue Brief2005,10(7):1～4.
  • 2Landis S H,Murray T,Bolden S,et al.Cancer statistics[J].Cancer J Clin,1998,48(6～29):43～62.
  • 3Nakazato T,Ito K,Miyakawa Y,et al.Catechin,a green tea component,rapidly induces apoptosis of myeloid leukemic cells via modulation of reactive oxygen species production in vitro and inhibits tumor growth in vivo[J].Haematologica,2005,90(3):317～325.
  • 4Kinlen,L J,Willows A N,Goldblatt P,et al.Tea consumption and cancer[J].Brit J Cancer,1988(58):397～401.
  • 5Heilburn L K,Nomura A,Stemmermann,G N.Black teaconsumption and cancer risk:A prospective study[J].Br J Cancer,1986(54):677～683.
  • 6Wynder E L,Rose D P,Cohen L A.Nutrition and prostate cancer:a proposal for dietary intervention[J].Nutr Cancer,1994(22):1～10.
  • 7Hussain T,Gupta S,Adhami V M,et al.Green tea constituent epigallocatechin-3-gallate selectively inhibits COX-2 without affecting COX-1 expression in human prostate carcinoma cells[J].Int J Cancer,2005,113(4):660～669.
  • 8Hiipakka R A,Zhang H Z,Dai W,et al.Structure-activity relationships for inhibition of human 5alpha-reductases by polyphenols[J].Biochem Pharmacol,2002,63(6):1165～1176.
  • 9Brusselmans K,De Schrijver E,Heyns W,et al.Epigallocatechin-3-gallate is a potent natural inhibitor of fatty acid synthase in intact cells and selectively induces apoptosis in prostate cancer cells[J].Int J Cancer,2003,106(6):856～862.
  • 10Pezzato E.,Sartor L,Dell'Aica I,et al.Prostate carcinoma and green tea:PSA-triggered basement membrane degradation and MMP-2 activation are inhibited by (-)epigallocatechin-3-gallate[J].Int J Cancer,2004,112(5):787-792.

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茶叶科学
2007年 第4期

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