丹尼斯凤梨叶片总RNA提取方法的比较

Comparsion of Different Methods of RNA Isolation from Guzmania ‘Denise’ Leaf

以丹尼斯凤梨(Guzmania‘Denise’)叶片为材料,利用Trizol试剂快速提取法、异硫氰酸胍法、苯酚法和CTAB法提取丹尼斯凤梨总RNA,通过RNA的产率、纯度、电泳图等分析来确立适用于丹尼斯凤梨总RNA分离的方法。结果表明,Trizol法提取的RNA具有28SrRNA、18SrRNA和5SrRNA3条较清晰的条带,很少有降解,具有较高的纯度,其他3种方法获得的RNA纯度较低、降解严重,有较多小分子和盐存在,RNA无明显条带出现,而是以小分子RNA弥散状分布。

A suitable method of RNA isolation from Guzmania 'Denise' was established through analyzing the rate of yield and purity of total RNA which was obtained by the methods of Trizol regent,guanidine thiocyanate,phenol and CTAB.The results indicated that the RNA isolated by Trizol have three clear bands of 28S rRNA,18S rRNA and 5S rRNA respectively,and its purity was higher than those of the other methods though its yield was lower slightly.RNA isolated by other methods degrades seriously and disperse.