高效液相色谱法测定清热止泻合剂中葛根素的含量

Content determination of puerarin in Qingre-zhixie Oral Liquid by HPLC

目的：测定清热止泻合剂中葛根素的含量。方法：采用HPLC法（C18柱）;流动相为甲醇-水（25∶75）;流速1.0ml/min;检测波长250nm。结果：葛根素在15.68-196μg/ml浓度范围内线性关系良好（r=0.9999）;平均回收率为98.7%,RSD=0.84%。结论：所建立的含量测定方法简便,准确,重现性好,可作为该产品的质量控制方法。

Objective：To establish a method to determine the content of puerarin in Qingre-zhixie Oral Liquid.Methods： HPLC instrument with C18 chromatographic column was used.The mobile phase was methanol-water（25：75）with 1.0 ml/min of flow rate.The wavelength for detection was 250 nm. Results： The linear range of puerarin was 15.68-196 μg/ml（r=0.999 9）. The average recovery was 98.7%. RSD was 0.84%.Conclusion： This method is simple,sensitive,accurate and reproducible, which can be used in the quality control of this preparation.