用RT-PCR方法检测颞叶癫痫脑内SH3GL2基因的表达

Detection of intracerebral SH3GL2 gene expression in human temporal lobe epilepsy by RT-PCR technique

目的研究难治性颞叶癫痫患者脑组织中的SH3GL2mRNA的表达,从分子水平探讨难治性颞叶癫痫发病的可能机制。方法运用RT-PCR检测SH3GL2mRNA在10例难治性癫痫患者及10例正常对照组脑部的表达。结果利用RT-PCR检测SH3GL2mRNA在难治性癫痫患者脑部表达增强,其电泳条带目的片断值与对照组比较差异有统计学意义(P<0.01)。结论中枢神经系统的SH3GL2在难治性癫痫患者脑部表达增强,可能在人类难治性癫痫的发病机制中起到一定作用。

Objective To detect intracerebral expression of SH3GL2mRNA in human temporal lobe epilepsy （TLE） by RT-PCR, and explore the possible pathogenic： mechanisms of refractory TLE at the molecular level. Methods SH3GL2 expression in the brain of 10 TLE patients and 10 normal controls was assayed by reverse transcription polymerase chain reaction （RT-PCR）. Results： The results of RT-PCR showed that SH3GL2 expression increased significantly in the TLE patients, as compared with the normal controls. Conclusions SH3GL2 expression was enhanced in the central nervous system of patients with refractory TLE, which may be one of the underlying causes in the development and progression of human refractory. TLE.