剪应力和周向应变联合作用下血管内皮细胞内自由Ca^(2+)响应

The Cytosolic-Free Calcium Response in Vascular Endothelial Cells Simultaneously Exposed to Shear Stress and Circumferential Stretch

机械力调节血管内皮细胞功能。Ca2+在机械力信号转导中扮演了重要的角色。本文研究剪应力和周向应变联合作用下血管内皮细胞内自由Ca2+浓度的变化规律,结果表明,在生理周向应变条件(小于15%)下,同时暴露于剪应力和周向应变的细胞内自由Ca2+浓度变化更依赖于剪应力大小而非周向应变的大小,Ca2+浓度升高主要是胞外Ca2+内流引起的。

Mechanical forces mediate the function of vascular Endothelial Cells （ECs）. Ca^2＋ plays an important role in mechanotransduction. The cytosolic-free Ca^2＋ response in ECs simultaneously exposed to shear stress and circumferential stretch was investigated. It was demonstrated that under physiological circumferential stretch （less than 15%）, the changes in the cytosolic-free Ca^2＋ concentration in ECs simultaneously exposed to shear stress and circumferential stretch were dependent on shear stress rather than circumferential stretch. The elevation in Ca^2＋ concentration was induced by extracellular Ca^2＋ influx.