摘要
目的观察胆红素脑病仔鼠血液神经元特异性烯醇化酶(NSE)、S-100蛋白(S-100β)、总胆红素含量(TBC)的变化及亚低温的脑保护作用。方法出生7 d的Wistar仔鼠42只随机分为C组(对照组,n=10)、M0组(未干预组,n=17)和M1组(亚低温组,n=15);C组给予腹腔注射生理盐水0.5 ml,其余各组腹腔注射胆红素200 mg/kg制成胆红素脑病模型。通过病理和血液NSE、S-100β值的变化,观察亚低温对胆红素脑病仔鼠的脑保护作用。结果胆红素脑病新生仔鼠出现明显的神经行为异常和病理改变,M1组经亚低温干预后血液NSE、S-100β、TBC下降,与M0组相比差异有非常显著性意义(P<0.01)。结论亚低温对胆红素脑病仔鼠有脑保护作用。
Objective To observe the changes of neuron-specific enolase (NSE), S-100 protein and TBC in the blood of newborn rats at early stage of bilirubin eneephalopathy, and the protective effect of mild-hypothermia on the brain. Methods 42 Wistar rats (7-day postnatal) were divided randomly into the group C (control group, n= 10), group M0 (normal-temperature group, n= 17) and group M1 (mild-hypothermia group, n= 15). The rats of the group C received physiological saline 0.5 ml, the rats in the groups of M0 and Mi were injected with bilirubin intraperitoneally (200 mg/kg) to establish the model of bilirubin eneephalopathy. The changes of the content of NSE and S-100 protein in the blood of newborn rats, and the protective effect of mild-hypothermia on brain were observed. Results The animals with established bilirubin eneephalopathy shown significant changes of neurobehaviour and path- ologieal examination. Values of NSE and S-100 protein of the group M1 decreased after the treatment of rnild-hypothermia, and there was a significant difference compared with group M0 ( P d0.01). Conclusion The mild-hypothermia has protective effect on brain of newborn rats with bilirubin intraperitoneally.
出处
《中国康复理论与实践》
CSCD
2007年第10期928-930,共3页
Chinese Journal of Rehabilitation Theory and Practice