亚低温对胆红素脑病仔鼠脑保护作用的实验研究

Protective Effect of Mild-hypothermia on Brain of Newborn Rats with Bilirubin Encephalopathy

目的观察胆红素脑病仔鼠血液神经元特异性烯醇化酶(NSE)、S-100蛋白(S-100β)、总胆红素含量(TBC)的变化及亚低温的脑保护作用。方法出生7 d的Wistar仔鼠42只随机分为C组(对照组,n=10)、M0组(未干预组,n=17)和M1组(亚低温组,n=15);C组给予腹腔注射生理盐水0.5 ml,其余各组腹腔注射胆红素200 mg/kg制成胆红素脑病模型。通过病理和血液NSE、S-100β值的变化,观察亚低温对胆红素脑病仔鼠的脑保护作用。结果胆红素脑病新生仔鼠出现明显的神经行为异常和病理改变,M1组经亚低温干预后血液NSE、S-100β、TBC下降,与M0组相比差异有非常显著性意义(P<0.01)。结论亚低温对胆红素脑病仔鼠有脑保护作用。

Objective To observe the changes of neuron-specific enolase （NSE）, S-100 protein and TBC in the blood of newborn rats at early stage of bilirubin eneephalopathy, and the protective effect of mild-hypothermia on the brain. Methods 42 Wistar rats （7-day postnatal） were divided randomly into the group C （control group, n= 10）, group M0 （normal-temperature group, n= 17） and group M1 （mild-hypothermia group, n= 15）. The rats of the group C received physiological saline 0.5 ml, the rats in the groups of M0 and Mi were injected with bilirubin intraperitoneally （200 mg/kg） to establish the model of bilirubin eneephalopathy. The changes of the content of NSE and S-100 protein in the blood of newborn rats, and the protective effect of mild-hypothermia on brain were observed. Results The animals with established bilirubin eneephalopathy shown significant changes of neurobehaviour and path- ologieal examination. Values of NSE and S-100 protein of the group M1 decreased after the treatment of rnild-hypothermia, and there was a significant difference compared with group M0 （ P d0.01）. Conclusion The mild-hypothermia has protective effect on brain of newborn rats with bilirubin intraperitoneally.