雌激素受体α与其靶基因LRP16相互作用的研究

Study on the interaction of estrogen receptor α and its target gene LRP16

目的:既往研究表明LRP16是雌激素受体α(ERα)诱导表达的一个靶基因,但可以反馈激活ERα介导的转录活性,本研究目的在于探讨该反馈效应的分子机制。方法:GST-pulldown与免疫共沉淀(CoIP)方法检测LRP16与ERα体内外的相互作用。结果:GST-pulldown与CoIP实验结果均表明LRP16与ERα可以直接结合,并且表明该相互作用不依赖于雌激素的存在,但雌激素可以增强二者的结合。结论:LRP16不仅是ERα的一个下游靶基因,而且是ERα的一个共激活因子。

Objective：LRP16 is an estrogen receptor （ERα） target gene, and the ERα-mediated transactivation is enhanced by LRP16. The study is to investigate the molecular mechanism underling this feedback effect. Methods： The in vitro and in vivo interactions of ERα and LRP16 were detected by GST-pulldown and coimmunoprecipitation （CoIP） assays, respectively, gesults：LRP16 could directly bind to ERα in an estrogen-independent manner, but could be enhanced by estrogen. Conclusion： LRP16 is not only an estrogen-induced gene by ERα, but also acts as a coactivator of ERα.