前列腺素E_1对大鼠弥漫性脑损伤后脑组织中TNFα和IL-1β的影响

Effects of prostaglandin E_1 on TNFα and IL-1β of thebrain following diffuse brain injury in rats

目的:研究前列腺素E1(PGE1)对减轻颅脑创伤后脑组织损伤机制。方法:取雄性SD大鼠35只,随机分为假手术组、生理盐水对照组、小剂量治疗组、中等剂量治疗组、大剂量治疗组5组,每组各7只。采用Marmarou A脑损伤模型,造成大鼠弥漫性脑损伤(DBI),观察PGE1对大鼠DBI后8 h脑组织中肿瘤坏死因子(TNFα)和白细胞介素-1β(IL-1β)含量的影响。结果:中小剂量PGE1治疗后脑组织TNFα和IL-1β含量明显减少,与NS对照组比较有显著性差异(P<0.05,P<0.01),与假手术组比较无差异(P>0.05);大剂量PGE1治疗前后脑组织TNFα和IL-1β含量无改变,与NS对照组比较无差异(P>0.05),与假手术组比较有非常显著性差异(P<0.01)。结论:中小剂量PGE1能降低颅脑损伤后炎症反应,减轻脑组织损伤,对颅脑损伤有一定的脑保护作用。

Objective ：To studay the protective effect of prostaglandin E1 （PGE1） on brain in experimental brain injury. Methods ：Thirty-five male SD rats were divided randomly into five groups：pseudoperation group, NS control group, Small-dose treat group, medium-dose treat group and larger-dose treat group. Each group consisted of 7 rats. Based on Marmarou A model of brain injury,we made rats model with diffuse brain injury（DBI） and observed PGE1＇s effect on tumor necrosis factor-alpha （TNFα） and interleukin-1 beta（IL-1β） contents at 8h after operation or DBI. Results：After treating with small and medium dose of PGE1, TNFα and IL-1β decreased remarkably,and there was a remarkable difference compared with NS control group （ P 〈 0.05, P 〈 0.01 ）. There was no difference compared with pseudoperation group （ P 〉 0.05）. Larger dose of PGE1 group had no effect on TNFα and IL-1β contents, and had no difference compared with NS control group（ P 〉 0.05 ）, but there was a significant difference compared with pseudoperation group（ P 〈0.01 ）. Conclusion：The inhibition of injury induced inflammation response in the brain tissue by small and medium dose of PGE1 may contribute to its overall neuroprotective action.