分泌型重组人粒细胞-巨噬细胞集落刺激因子中试发酵工艺的研究

Pilot Fermention Process of Secretory Recombinant Human Granulocyte/Macrophage Colony Stimulating Factor

通过对GM－CSF工程菌W3100发酵条件的研究，优化了培养及表达条件，使工程菌W3100在30L发酵罐可表达分泌型GM－CSF1.5g／L左右，占菌体总蛋白的25％以上，纯化后的比活大于2．0×107。

The culture and expression coditions of secretory recombinant E. coli W3100/pGM-CSF were optimized during the pilot fermention process. More than 25 % (about 1. 5g/L) of total somatic proteins of secrtory GM-CSF were expressed in W3100 culture in 30L fermenter under the improved conditions. The specific activities of product reached over 2.0×107/ mg protein after being purified,