摘要
将合链霉亲和亲-蛋白A(Streptavidin-proteinA)融合蛋白基因的表达质粒pTSAPA导入大肠杆菌BL21(DE3),成功地表达了该融合蛋白,经SIJS-PAGE及免疫印迹等方法证实该蛋白既具有IgG的结合活性,又具生物素结合活性。
Streptavidin-protein A chimeric protein was successfully expressed by transforming the plasmid (pTSAPA) carrying the gene coding the protein into a E. coli strainBL21 (DE3). SDS-PAGE and Western blot showed binding abilities of the expressed proteinto both IgG and biotin.
出处
《中国生物制品学杂志》
CAS
CSCD
1997年第2期75-78,共4页
Chinese Journal of Biologicals