胎盘、脐血和骨髓来源的贴壁细胞的生物学特性的比较

Comparison of the adherent cells derived from human placenta,umbilical cord blood and bone marrow

目的探讨人胎盘、脐血和骨髓来源的贴壁细胞的分离培养和生物学特性,为间充质干细胞(MSC)的选择和应用提供依据。方法采用酶消化法分离人胎盘组织,60 g/L羟乙基淀粉和密度梯度离心两步法分离脐血单个核细胞,密度梯度离心法分离骨髓单个核细胞,分别进行贴壁培养,观察不同来源MSC的生长、增殖和表面标志的表达并做比较。结果胎盘来源的贴壁细胞为一种混合性细胞,脐血单个核细胞体外培养可以获得形态不均一的贴壁细胞,二者在生长规律和形态学特征上与骨髓基质相比有一定差异性,CD106、CD44在三种细胞均有表达。结论胎盘和脐血来源的贴壁细胞具备间充质干细胞的基本特征。

Objective To compare the adherent cells derived from human placenta, umbilical cord blood and bone marrow, and provide laboratory data for clinical application of mesenchymal stem cells. Methods Adherent cells were isolated from human placenta tissues by enzyme digestion, and mononuclear cells （MNC） were isolated from umbilical cord blood （UCB） by 60g/L HES and density gradient centrifugation and MNC were isolated from bone marrow （BM） by density gradient centrifugation, and then these cells were cultured in vitro. Their biological characteristics were studied and compared. Results The adherent cells cultured from human placenta and umbilical cord blood had disparate shape in vitro respectively. And they had some differences in growth and shape from those derived from bone marrow. The adherent cells derived from the three tissues all expressed CD106 and CD44 in immunohistochemistry staining. Conclusion The adherent cells derived from human placenta and umbilical cord blood have the basic features of mesenchymal stem cells.