丹参酮ⅡA对人胃腺癌细胞SGC-7901增殖、凋亡的影响

The effect of Tanshinone ⅡA on proliferation and apoptosis of human gastric adenocarcinoma cell line SGC-7901

目的体外实验检测丹参酮ⅡA(TanⅡA)对SGC-7901人胃腺癌细胞增殖、凋亡的影响,探讨其可能的作用机制。方法体外常规培养SGC-7901人胃腺癌细胞用于实验,经TanⅡA作用后,采用MTT比色法检测细胞增殖情况,电镜及流式细胞仪观察胃腺癌细胞凋亡及细胞周期分布。结果TanⅡA对胃腺癌细胞的生长有明显的抑制作用,呈剂量依赖性,与对照组相比差异有统计学意义(P<0.01);TanⅡA作用后胃腺癌细胞表现为凋亡特征性的形态改变;TanⅡA可时间依赖性地诱导肿瘤细胞凋亡。结论TanⅡA对体外培养的SGC-7901人胃腺癌细胞的生长有明显的抑制作用和诱导凋亡的作用,其可能的机制为TanⅡA可将细胞阻滞于G0/G1期,使其不能进入S期。

Objective To observe the effect of Tanshinone Ⅱ A on proliferation and apoptosis of human gastric adenocarcinoma cell line SGC-7901 and its mechanism. Methods The human gastric adenocarcinoma cell line SGC-7901was cultured in vitro and with Tanshinone Ⅱ A at various concentrations. Cytotoxicity was valuated by MTT assay, apoptosis-related alterations in morphology were ascertained by transmission electron microscopy. The cell cycle distribution and the apoptotic rate were investigated by flow cytometry. SGC-7901 cell apoptosis with nuclear chromatin concentration and fragmentation as well as cell shrinkage and the formation of apoptotic bodies were observed by TEM. Results The growth of SGC-7901 cells was inhibited significantly in a dose-dependent manner. Tanshinone Ⅱ A could induce the apoptosis in time-dependent manner. Oonclusion Tanshinone Ⅱ A has effect in inhibiting SGC-7901 cells and inducing the apoptosis. The apoptotic rate and percentage of cells in G1 phase increased in the SGC-7901 cells treated by Tanshinone Ⅱ A.