P物质-聚乳酸-聚乙醇酸共聚物纳米缓释微粒的制备及其对人牙周膜细胞的作用的研究

Preparation of substance P-loaded PLGA nanoparticles and investigation of their effects on proliferation of PDLCs in vitro

目的制备P物质-聚乳酸-聚乙醇酸共聚物(PLGA)纳米缓释微粒,考察纳米粒一般性质、P物质生物活性保存情况,以及它对人牙周膜细胞(PDLCs)的作用。方法以PLGA为载体,运用复乳-溶剂挥发法制备P物质缓释纳米粒,将其加入PDLCs的培养液中,运用四甲基偶氮吐盐微量反应比色法(MTT)测定细胞增殖情况。实验分为P物质组、缓释纳米粒组以及对照组。结果P物质缓释纳米微粒球体均匀度好,平均粒径(114.56±21.42)nm。培养第1天时,各组吸光度(A)值差异均无显著性;第3天时,P物质组A值大于另外两组,5d后缓释纳米粒组A值高于另外两组。结论建立了较好的P物质PL-GA纳米缓释微粒粉制备工艺,纳米粒中P物质生物活性保存良好,能持续释放P物质,较长时间促进成纤维细胞增殖。

Objective To prepare SP-loaded PLGA nanoparticles and investigate their general properties,preservation of SP bioactivity and their effects on human PDLCs. Methods With PLGA as carrier, SP drug delivery nanoparticles were prepared by double emulsion-solvent e- vaporation method, The proliferation of PDI_Cs was observed by MTI＂ method after SP-loaded PLGA nanoparticles were added into the culture medium. Results The SP-loaded PLGA nanoparticles were spherical, with mean diameter of 114.56 ± 21.42 um. The celluar study showed no significant difference in A value among the three groups on the first day of plate culture; the A value of the SP group was higher than that of the other two groups on the third day of plate culture; the A value in the SP-loaded PLGA nanoparticles group was the highest after 5 days of plate culture. Conclusion The preparation method of the SP-loaded PLGA nanoparticles was established and the bioactivity of SP in the nanoparticles was well preserved. The SP-PLGA hat,particles can release SP continuously and promote the proliferation of PDLCs in vitro for a relatively longer time.