不同方法提取的复方芍芪多苷对小鼠免疫性肝损伤保护作用比较

Comparison of Protective Effect of Shaoqiduogan Extracted by Different Methods on Immunological Liver Injury in Mice

目的:比较不同方法提取的复方芍芪多苷(SQDG)对小鼠免疫性肝损伤的保护作用。方法:在建立卡介苗+脂多糖诱导免疫性肝损伤小鼠模型的基础上,用分光光度法检测血清中丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)水平和肝匀浆丙二醛(MDA)、谷胱甘肽过氧物酶(GSH-PX)、超氧化物歧化酶(SOD)含量。结果:SQDG中剂量组与白芍总苷(TGP)+黄芪总皂苷(ASTs)中剂量组相比较,在降低小鼠血清中升高的ALT,AST水平及降低肝匀浆MDA水平上,组间差异具有显著性,对其余指标的影响无显著性。结论:SQDG120 mg/kg和TGP 105 mg/kg+ASTs 15 mg/kg对免疫性肝损伤小鼠模型具有一定的药效学差异。

Objective： To compare the protective effects of Shaoqiduogan （SQDG） extracted using different methods on immunological liver injury in mice. Methods： The model of immunological liver injury in mice was induced by injection of Bacillus Calmette Guerin（ BCG ） and lipopolysaccharide （LPS） from tail vein. The alanine aminotransferase （ALT）, aspartate aminotransferase （AST）, malondialdehyde （MDA） content, superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px） activities in liver homogenate were assayed by spectrophotom etry. Results： Compared with total glucosides of paeony （TGP） 105 mg/kg ＋ total saponin of astragalus mongholicus （ASTs） 15 mg/kg, SQDG 120 mg/kg significantly decreased the serum transaminase levels, MDA production, while had no significant effects on other indices. Conclusion： SQDG 120 mg/kg and TGP 105 mg/L ＋ ASTs 15 mg/kg have different pharmacological effects on the model of immunological liver injury in mice.