诱骗受体3对大鼠移植肝脏的保护作用

Protective effects of decoy receptor 3 on rat liver allograft

目的将重组诱骗受体3（decoy receptor 3，DcR3）腺相关病毒（adeno-associated virus，AAV）感染移植肝，探讨DcR3对移植肝的保护作用。方法实验动物分为同基因肝移植组（G1）、同种异体肝移植组（G2）、AAV空病毒感染的同种异体肝移植组（G3）、重组DcR3／AAV感染的同种异体肝移植组（G4）。常规建立同种异体大鼠肝移植模型；术中取预先制备的浓缩1×10^9IU病毒颗粒感染移植肝；术后观察大鼠的存活时间、肝功能，荧光显微镜及光镜下观察DcR3在肝脏中的表达及肝脏病理改变。结果G4组的平均生存时间比G2、G3组显著延长，G2、G3组的平均生存时间差异无统计学意义。G4组大鼠术后15、20d的肝功能、肝脏病理改变分别与G2、G3组大鼠术后7、10d相似。结论DcR3对大鼠移植肝有显著的保护作用。

Objective To investigate the protective effect of decoy receptor 3 （DcR3） on rat liver allograft by infecting liver allograft with recombinant human DcR3/adeno-associated virus （AAV）. Methods All rats were divided into isogenic liver transplantation group （ G1 ）, allogenic liver transplantation group （ G2）, allogenic liver transplantation group infected by AAV virus （G3） and allogenic liver transplantation group infected by recombinant DcR3/AAV （G4）. Rat liver allotransplantation model was established by regular methods. Liver allografts were infected by condensed viral particles （ 1 × 10^9 IU） perioperatively. Survival time of rates and liver function were investigated, and the expression of DcR3 in liver allograft and pathological changes of liver were ob- served under fluorescent microscope and light microscope. Results The mean survival time of rats in G4 was significantly longer than that in G2 and G3, with no statistical difference between G2 and G3. The pathological changes of liver and liver function at postoperative days 15 and 20 of G4 were similar to those at postoperative days 7 and 10 of G2 and G3. Conclusions DcR3 has protective effect on rat liver allograft after liver transplantation.