T淋巴瘤侵袭转移诱导因子1与胃癌细胞侵袭移行能力的关系

Correlation between invasion and migration ability of gastric cancer cells and Tiam1

目的检测胃癌细胞中T淋巴瘤侵袭转移诱导因子1（T lymphoma invasion and metastasis inducing factor1，Tiaml）的表达及其与肿瘤侵袭、移行能力的关系，同时观察胃癌细胞在形态学方面的变化。方法采用层黏连蛋白黏附法，由胃癌MKN-45细胞株（M0）中筛选获得高（MH）、低（ML）黏附亚株。应用流式细胞仪检测Tiaml在胃癌细胞中的表达，以Boyden小室法测定M0、ML、MH细胞的体外侵袭、移行能力，并分析其与Tiaml表达间的关系，同时采用苏木精-伊红及细胞骨架蛋白染色、扫描电镜技术观察胃癌细胞形态。结果MH细胞的体外侵袭、移行能力及Tiaml表达均强于M0、ML细胞（P〈0．05），且Tiaml表达与胃癌细胞体外侵袭、移行能力呈正相关（r=0．997和1．000）；同时与M0、ML相比，MH胞体伸展，表面突起增多，片状伪足宽厚，丝状伪足细长而密集，异型性明显，且细胞骨架结构较紊乱，斑点状肌动蛋白小体增多。但M0、ML细胞间差异无统计学意义（P〉0．05）。结论Tiaml表达水平升高可能促进胃癌细胞侵袭、移行。这或许是通过调整胃癌细胞骨架结构重组，增强其变形、游走能力而实现的。

Objective To investigate the expression of T lymphoma invasion and metastasis inducing factor 1 （Tiaml） in gastric cancer cells and its relationship with the invasion and migration ability of gastric cancer cells, and observe the morphological changes of gastric cancer cells. Methods High adhesive subgroups （ MH ） and low adhesive subgroups （ ML） were separated from MKN45 cell strain （ M0 ） by laminin adhesion method in vitro. The expressions of Tiaml in Mo, ML and Mn cells were detected by flow cytometry, the invasion and migration ability of M0, ML and M, cells in vitro by Boyden chamber. The correlation between invasion and migration ability of M0, ML, M. cells and expression of Tiaml in M0, ML, Mn cells was analyzed. The morphological differences among M0, ML and MH cells were observed by hematoxylin eosin stain, cytoskeletal protein stain and scanning electronic microscope. Results The invasion and migration ability and the expression of Tiaml in MH cells were higher than those in M0 and ML cells （ P 〈 0.05）. The expression of Tiaml was positively correlated with the invasion and migration ability of gastric cancer cells （ r = 0. 997, 1. 000）. Compared with the M0 and ML cells, the MH cell bodies elongated with wide and thick lamellate pseudopodia, slender and intensive filamentous pseudopodia, more projections on the surface, derangement of cell skeleton and more dot-like actin bodies. There was no significant difference between M0 and ML cells （ P 〉 0.05 ）. Conclusions The high expression level of Tiaml may promote the invasion and migration of gastric cancer cells through adjusting the cytoskeletal reconstruction and enhancing the deforming and migration ability of gastric cancer cells.