RAPD技术在甜高粱品种基因组分析上的应用

Analysis of Sweet Sorghum Genome by Using RAPD

以7050B保持系(抗病)、TX622B保持系(感病)杂交的F2抗性个体和甜高粱丝黑穗病8113(抗病)、甜高粱丝黑穗病8101(感病)为试材,应用RAPD筛选技术对甜高粱丝黑穗病感、抗病基因组进行分子标记的初步分析.实验过程中采用CTAB法提取高粱、甜高粱基因组总DNA,应用琼脂糖凝胶电泳法对RAPD扩增结果进行检测,通过比较琼脂糖凝胶电泳电压的不同,进一步优化了琼脂糖电泳电压,以7050B保持系(抗病)、TX622B保持系(感病)杂交的子一代抗性个体为试材筛选了90个引物,其中29引物扩增出了多态性谱带,应用20个具有多态性扩增谱带的引物对甜高粱丝黑穗病8113(抗病)、甜高粱丝黑穗病8101(感病)进行了RAPD分析,结果表明共有9个引物扩增出了差异谱带,引物分别为S83,S-84,S-88,S-89,S-90,S-92,S-96,S-97,S-98.

Sorghum of 7050B（resistant parent against head smut） TX622B （susceptible parent against head smut） and sweet sorghum of 8113（resistant parent against head smut） 8101（susceptible parent against head smut） were studied by RAPD. The method of DNA extracting and purifying were elected and optimized. It is CTAB method. 90 random sequence dicer-primer were used to screen, the samples for markers linked to the head smut resistance C-ene of Sorghum and Sweet Sorghum. Under this experiment condition, 29 random sequence dicer-primer were high efficient bands of DNA were amplified,＇ Polymorphism bands were amplified with primer S-83, S-84, S-88, S-89, S-90, S-92, S-96, S-97, S-98.