高效毛细管电泳法测定麦当乳通颗粒剂中阿魏酸

Determination of Ferulic Acid in "MAI-DANG-RU-TONG" Granules by High Performance Capillary Electrophoresis

提出了用高效毛细管区带电泳法（CZE）测定中成药麦当乳通颗粒中阿魏酸含量的方法。电泳分离在控温18℃的无表面涂层的熔质石英毛细管中进行。以12．5mmol·L^-1硼砂缓冲液（pH8．20）为运行缓冲溶液，压力进样（6kPa×3s），检测波长为200nm。在1～52mg·L^-1质量浓度范围内呈线性（r=0．9999），回收率为98．77％，RSD为1．9％，日内和日间的相对标准偏差分别为1．9％和1．6％。

Ferulic acid in a ready-made Chinese herbal medicine, called ＂MAI-DANG-RU-TONG＂ （in granules） was determined by high performance capillary zone electrophoresis （HPCZE）. Electrophoretic separation was performed on an uncoated fused-silica capillary at 18 ℃. A 12. 5 mmol·L^-1 borax buffer solution of pH 8. 20 was used as moving buffer. Sample introduction was conducted hydrodynamically at 6 kPa for 3 s, with detection wavelength at 200 nm. Linearity was kept in the concentration range of 1 - 52 mg·L^-1 of ferulic acid with correlation coefficient of 0. 999 9. Average recovery of 98. 77% and RSD value of 1.9% were obtained. Values of intra-day and inter-day RSD were found to be 1.9 % and 1.6 % respectively.