应用巢式PCR法鉴定牛早期胚胎性别

Using Nested PCR for Sex Determination of Bovine Early Embryos

为获得一种高效的牛胚胎性别鉴定方法,采用高特异性和灵敏度的巢式PCR进行扩增。结果表明:公牛可以扩增出187bp的SRY基因片段和255bp的β-珠蛋白基因片段,母牛只能扩增出255bp的β-珠蛋白基因片段。巢式PCR只需3～8个细胞就可以观察到扩增结果,而常规PCR则需要较多的细胞,所以牛胚胎性别鉴定时使用巢式PCR效果更好。

In this experiment, in order to obtain a high efficiency sex determination method, we used nested PCR to amplify purpose fragments. The result showed that 187 bp and 255 bp bands could be visible on the gel in ultraviolet transilluminator for male, but for female, only 255 bp band could be visible. It needed only 3 to 8 cells for obtaining amplification result by nested PCR, but more cells were needed by normal PCR. So we could obtain better results of sex determination of bovine preimplantation embryos by nested PCR.