乙型肝炎病毒前C基因区变异与乙肝病毒复制关系的临床研究

Clinical research of the relationship between mutations of hepatitis B virus precore and the core promoter gene and HBV DNA concentration

目的探讨乙型肝炎病毒(Hepatitis B Virus,HBV)前C基因区变异与HBV-DNA载量的关系。方法通过DNA扩增、基因序列分析检测21例慢性肝炎、18例肝硬化和15例肝癌血清的HBV前C区和基本核心启动子(Basic Core Promoter,BCP)基因序列,荧光定量聚合酶链反应技术定量检测血清中的HBV-DNA。结果野生株与前C区终止变异、BCP双变异以及联合变异组HBV-DNA载量测定差异无显著性(P>0.05);BCP双变异HBV-DNA载量HBeAg(-)组显著高于HBeAg(+)组(P>0.05)。结论前C区终止变异和BCP双变异对HBV DNA复制无明显影响。HBeAg(-)的慢性肝病患者BCP变异后HBV DNA复制明显活跃。

Objective To investigate the relationship between mutations of hepatitis B virus （HBV） precore and basic core promoter （BCP） gene and HBV DNA concentration. Methods HBV DNA fragments in the CP and precore gene regions were amplified by PCR, and sequenced directly from sera of 54 cases with chronic hepatitis B, 18 cases with liver cirrhosis and 15 cases with hepatocellular carcinoma. The quantities of serum HBV DNA were detected by using fluorescent quantitative-PCR technology. Results Precore mutation and double mutations did not significantly affect HBV DNA concentration（P 〉0.05）. The HBV DNA concentration of double mutations was significantly higher in HBeAg negative group than HBeAg positive group （P 〉 0. 05）. Conclusion The replication of HBV DNA with CP double mutations in HBeAg positive group is remarkablely lower than HBeAg negative group.