高效液相色谱法测定盐酸洛美沙星葡萄糖注射液的含量

HPLC for concentration determination of lomefloxacin hydrochloride and glucose injection

目的：探索准确而简便地测定盐酸洛美沙星葡萄糖注射液的含量。方法：采用高效液相色谱法，用硅胶柱，以十二烷基硫酸钠磷酸二氢钾混合溶液甲醇（６０∶４０）为流动相，在波长２８７ｎｍ处进行检测。结果：在５．５５～１１１．００μｇ·ｍｌ－１的浓度范围具有良好的线性关系，ｒ＝０．９９９８，平均回收率９９．５９％（ｎ＝５），ＲＳＤ＝０．５４％。结论：本法适用于成品检验及杂质或降解产物的检查。

OBJECTIVE: To find a precise, simple and convenient method for concentration determination of lomefloxacin hydrochloride and glucose injection. METHOD: A HPLC method was chromatographed on silica columns with a mobile phase of a mixture solution of sodium dodecyl sulphate and potassium dihydrogen phosphate methanol(60∶40) with detection at wave length 287 nm. RESULTS: The linear range was 5.55～111.00 μg·ml -1 , the average recovery 99.59%, and RSD 0.54%(n=5). CONCLUSION: The method can be used to determine finished product and impurity.