OLFM4^(GW112/hGC-1)、GRIM19及PIN1基因在12种人肿瘤细胞株中的表达分析

Expression and significance of OLFM4^(GW112/hGC-1),GRIM19 and PIN1 genes in 12 human tumor cell lines

目的检测OLFM4GW112/hGC-1、GRIM19及PIN1基因在12种人肿瘤细胞株中的表达,并探讨与肿瘤细胞凋亡与周期的关系。方法利用RT-PCR半定量的方法检测GW112、GRIM19与PIN1三种基因在人12种肿瘤细胞株中转录水平。结果RT-PCR结果显示:12种人肿瘤细胞株中GW112、GRIM19与PIN1三种基因均有表达,未出现转录缺失。其中胃癌细胞SGC-7901与脑胶质瘤细胞CHG-5中的GW112表达明显强于GRIM19,而在其余十种细胞株中GW112表达却明显低于GRIM19。实验组细胞中,PIN1基因只在SGC-7901株与CHG-5株中表达较弱,其余表达均较强。结论GW112、GRIM19与PIN1在这些肿瘤细胞中的表达状况很可能与它们的周期调控和细胞凋亡有关,对三种基因的表达研究可进一步对分析三种基因的功能提供依据。

Objective To investigate expressions of OLFM4^GW112/hGC-1 ,GRIM19 and PIN1 genes in 12 different human tumor cell lines and to explore the significance in apoptosis and cell cycle. Method GW112,GRIM19,PIN1 and GAP- DH transcriptions in 12 kinds of human tumor cell lines were detected by RT-PCR. Result Using GAPDH as internal control,the expressions of GW112, GRIM19 and PIN1 were observed in 12 different human tumor cell lines. In human gastric cancer cell line SGC-7901 and human glioma cell line CHG-5, the expressions of GW112 was obviously higher than GRIM19. PIN1 and GRIM19 expressions in SGC-7901 and CHG-5 were obviously lower than those in other tumor lines. Conclusion The results indicate that the different expression models of GW112, GRIM19 and PIN1 genes may be related to the regulation of apoptosis and cell cycle in human tumor cells and could provide a perfect ideal for the further gene functional analysis.