摘要
目的分析南京儿童医院腹泻患儿中轮状病毒(RV)的感染情况。方法收集南京儿童医院2004年1月-2006年12月门诊及住院非细菌性感染性腹泻患儿粪便标本,经聚丙烯酰胺凝胶电泳(PAGE)或酶联免疫吸附试验(ELISA)检测RV。结果3 324例标本中共检出RV阳性1 406例,检出率为42.30%。其中6-11个月、1-2岁和2-3岁3个年龄段RV检出率分别为43.4%、47.1%和51.2%;RV腹泻年龄最高为6-11个月年龄段,达41.2%;全年检出RV比较,秋冬季(9-12月份)为检出高峰,占56.2%;1 035例RNA阳性标本中,RNA长型占819例(79.13%)。结论南京儿童医院RV腹泻中6个月-2岁婴幼儿是RV的易感人群。以秋冬季(9-12月份)为流行高峰,基因组以长型为主。
Objective To investigate the status of rotavirus(RV) infection among children with diarrhea in Nanjing Children's Hospital Methods Stool samples were collected from 3 324 children with nosocomially acquired diarrhea in Nanjing Children's Hospital of Nanjing Medical University between Jan. 2004 and Dec. 2006. Polyacrylamidegel electrophoresis ( PAGE ) and enzyme - linked immunosorbent assay (ELISA) were used to detect RV. Results Among 3 324 samples, 1 406 (42.30%) cases were detected positive for group A RV, and the high detection rate were found within 6 -11 months, 1 -2 years and 2 -3 years (43.4% ,47.1% and 51.2% ). Of all episodes of RV diarrhea, 82.2% occurred during the first 2 years of life,and 41.2% was found within 6 - 11 months. The peak point of RV diarrhea appeared in autumn - winter season ( September to December ). Among 1 035 cases positive for group A RV gene by PAGE, 819 cases (79.13 % ) showed long electrophe rotypes. Conclusions RV diarrhea is more prevalent among children of 6 months to 2 years old. Autumn - winter season ( september to december) is the epidemic season in Nanjing Children's Hospital.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2007年第19期1463-1464,共2页
Journal of Applied Clinical Pediatrics