川崎病患儿外周血单个核细胞中γ干扰素诱导蛋白10表达的意义

Significance of Expression of Interferon γ Inducible Protein 10 in Peripheral Blood Mononuclear Cells in Children with Kawasaki Disease

目的探讨川崎病(KD)患儿外周血单个核细胞(PBMC)中γ干扰素诱导蛋白-10(IP-10) mRNA表达意义。方法实时荧光定量反转录聚合酶链反应(real-time RT-PCR)检测42例KD及40例健康儿童PBMC中IP-10 mRNA表达。结果KD组PBMC中IP-10 mRNA表达水平为0.741±0.161,明显高于健康对照组0.388±0.063(t=12.988 P=0);KD冠状动脉损伤(CAL)亚组PBMC中IP-10 mRNA表达水平为0.825±0.142,高于无冠状动脉损伤(non-CAL)亚组0.708±0.158(t=2.233 P=0.031);静脉注射人免疫球蛋白(IVIG)治疗后IP-10 mRNA表达水平为0.421±0.119,较治疗前0.728±0.170下降(P<0.05),与健康对照组比较无显著性差异(P>0.05);未予IVIG治疗者常规治疗后IP-10 mRNA表达水平为0.674±0.140,较常规治疗前0.780±0.131轻度下降,但无显著性差异(P>0.05)。结论IP-10在KD患儿PBMC中表达增加,参与KD的发病过程,并与KD CAL发生有关,对PBMC中IP-10 mRNA表达抑制可能是IVIG治疗KD的作用机制之一。

Objective To explore the expression of interferon γ inducible protein 10 （ IP - 10） mRNA in peripheral blood mononuclcar cell（PBMC） in children with Kawasaki disease（KD） and its significance. Methods The expression of PBMC IP - 10 mRNA in 42 children with KD and 40 heahhy children were assayed by real - time reverse transcription polymerase chain reaction（ real - time RT - PCR）. Results The value ofIP- 10 mRNA in KD group was 0.741 ±0. 161 ,which was higher than that of control group 0. 388 ±0.063（t = 12.988 P=0）. The value of IP - 10 mRNA in coronary artery lesions （CAL） group was 0. 825 ±0. 142, which was higher than that non - coronary artery lesions （ non - CAL） group 0. 708 ±0. 158 （t = 2. 233 P = 0. 031 ）. The value of IP - 10 mRNA in KD after human immunoglobulin for intravenous injection （ IVIG ） treatment was 0. 421 ± 0.119 ,which was higher than the value before treatment 0. 728 ± 0.170 （P 〈 0.05 ）. hut there, was no significant difference compared to the control group（ P 〉 0.05 ）. The value of IP- 10 mRNA in KD without IVIG treatment was 0. 674 ±0. 141, and no significant difference compared with the value before rout treatment 0. 780 ± 0.131 （P 〉 0.05 ）. Conclusions The expression of PBMC IP - 10 mRNA increases in KD. IP - 10 may participate in the pathogenesis of KD and CAL in KD. Perhaps the inhibition to the expression of PBMC IP - 10 mRNA is one of the mechanisms of IVIG.