高脂饲料对C57BL/6J小鼠血脂及转氨酶的动态影响

Dynamic Observation of Serum Lipid and ALT Levels in C57BL/6J Mouse Models of Hyperlipidemia Induced by High-Lipid Diet

目的观察高脂饲料诱发的C57BL/6J小鼠高脂血症模型血脂的动态变化。方法C57BL/6J小鼠分成基础饲料组、高脂饲料组,在给予高脂饲料0、1、2、3、4、5、7、9、11、17周采血检测TC、TG、HDL-C、LDL-C、ALT、BUN,并且部分动物取心、肝、肾甲醛液固定,HE染色作病理学检查,剩余部分动物继续给予普通饲料,21周时采血检测血浆指标。结果给予高脂饲料后1周小鼠血脂和ALT显著升高,3～11周后稳定,此后稍有下降。BUN水平没有明显变化。心、肝脏显示明显病理学损伤,肾脏病理学检测正常。但是停止饲喂高脂饲料,小鼠血脂和ALT水平很快下降至正常。结论高脂饲料引起C57BL/6J小鼠血脂升高迅速、明显。

Objective To establish a hyperlipidemia animal model induced by high-fat diet, and observe the dynamic variation of the serum lipid levels at different time. Methods The C57BL/6J mice were randomly divided into 2 groups. The control group was fed with standard diet, the model group fed with high-fat diet. The serum TG, TC, HDL, LDL, ALT, albumin, and BUN were assayed at 0, 1, 2, 3, 4, 5, 7, 9,1 1, 17 weeks. Some mice were killed and the heart, liver and kidney were taken and HE-stained sections were prepared for histopathological examination. Then high-fat diet of the model group was changed to standard diet. After 4 weeks all mice were killed and the serum contents of each substance were detected. Results The levels of lipid and ALT in serum rose rapidly after 1 week of high-fat diet, and stay at a high level throughout the duration of 3-11weeks, then decreased a little. Pathological changes were observed in the heart and liver. The serum lipid and ALT levels decreased rapidly to normal level after stopping high-fat diet. Conclusion Feeding with high-fat diet can induce hyperlipidemia in mice rapidly and reliably.