α-生育酚对脑皮层神经元线粒体的保护作用

Protective Effect of α-Tocopherol on the Mitochondria of Cortex Neurons

目的探讨α-生育酚对神经元线粒体的保护作用。方法将神经元细胞进行分组,分为:(1)正常对照组,(2)单纯氧自由基损伤组,(3)α-生育酚保护组。利用Fenton反应造成神经元细胞氧自由基损伤,用激光共聚焦显微镜观察各组神经元JC-1染色结果,并检测各组神经元琥珀酸脱氢酶(SDH)和细胞色素氧化酶(CCO)活性。结果(1)JC-1染色结果分析:α-生育酚保护组神经元线粒体功能强于单纯氧自由基损伤组。(2)SDH和CCO酶活性分析:α-生育酚保护组神经元SDH和CCO酶活性高于单纯氧自由基损伤组。结论α-生育酚可以有效对抗氧自由基对神经元线粒体的损伤。

Objective To evaluate the protective effect of α-tocopherol on the mitochondria of cortex neurons. Methods Neurons were divided into 3 groups： （1） normal group, （2） group of oxidative damage, and （3） group under α-tocopherol protection. Oxidative damage was induced by Fenton reaction in groups （2） and （3）. The neurons were stained with JC-1 and examined the changes of mitochondria under confocal laser scanning microscope. The activity of SDH and CCO in the extracellular fluid was determined. Results 1. Analysis of JC-1 staining： α-tocopherol reduced the oxidative free radical-induced damage in mitochondria of neurons. 2. Analysis of SDH and CCO activity： The activity of SDH and CCO of group （3） was significantly higher than that of group （2）. Conclusion α-tocopherol can effectively protect the mitochondria of neurons from damage induced by oxidative free radicals.