卵丘细胞线粒体移植对年老小鼠早期胚胎发育的影响

Effect of Mitochondria Transplantation from Cumulus Granular Cells into Early Embryos of Ageing Mice

目的探讨线粒体对早期胚胎发育的影响。方法采用昆明小鼠为动物模型,以其卵丘颗粒细胞、卵母细胞、受精卵为试验材料,提取年老小鼠卵丘细胞线粒体,将其注入年老小鼠卵母细胞和体内冲出的受精卵中。结果与结论通过卵丘细胞线粒体移植,给卵子补充了一定量的线粒体,可以部分弥补卵母细胞因变异引起的功能不足。为第2次减数分裂、受精卵和胚胎发育提供足够的能量,明显改善了胚胎的质量。

Objective To assess the effect of mitochondria from cumulus granular ceils transplanted into early embryos of aging mice. Metheds Kunming mice were used as experiment animal model, and the cumulus granular cells, oocytes, zygotes were used as experimental material. Mitochondria were taken from cumulus granular ceils, purified and injected into oocytes and zygotes of ageing mice. Results The blastulation rate of ICSI group and ICSI ＋ MIT group were 24.98% and 32.54%, respectively, （P 〈 0.05）. The cleavage rate and blastulation rate in the sham injection group were 39.33% and 19.67%, those of MIT group were 67.90% and 51.54% , respectively. There was a significant difference between the sham injection and MIT groups （ P 〈 0.05）. Conclusion Mithochondria transplantation can improve the development potential of early embryos of ageing mouse.