饲料钙磷含量和钙磷比对生长期大鼠体重增长与骨骼发育的影响

Effect of Feed Calcium,Phosphorus and Calcium/Phosphorus Ratio on the Body Weight and Bone Development of Growing Rats

目的研究饲料中不同的钙磷含量和钙磷比对生长期实验大鼠体重及骨骼发育的影响。方法采用完全随机化设计方案,取1月龄(100±10)g清洁级Wistar雄性大鼠70只,随机分成7组,每组10只,饲喂7种不同钙磷含量的饲料。自由采食,饮用去离子水,试验期42 d。各组于实验开始和结束时称重。试验结束后,处死动物并分离一侧胫骨和股骨用于实验指标的检测。结果当保持饲料中钙或者磷中的一种含量不变的情况下,调整另一种的含量即改变钙磷比例时,1.2∶1的正常钙磷比例组生长期实验大鼠增重及骨骼发育要明显好于0.4∶1的低钙磷比例组和4∶1的高钙磷比例组(P<0.05),并且当保持饲料中钙磷比例不变时,即使在低钙低磷和高钙高磷进食水平下,正常钙磷比例组大鼠增重及骨骼发育都较好,但低钙磷比例组和高钙磷比例组大鼠增重及骨骼发育则由于饲料中钙磷含量的变化波动较大(P<0.05)。结论饲料中钙和磷需按比例添加,如果两者绝对含量相差过大就会影响到动物的增重及骨骼发育,本实验证明饲料钙磷比为1.2∶1时生长期实验大鼠增重及骨骼发育较好。

Objective To investigate the effect of feed calcium, phosphorus and calcium/phosphorus ratio on the body weight and bone development of growing rats. Methods 70 male Wistar rats aged 30 d were divided into 7 groups randomly according to their body weigh, 10 for each group was considered as one batch for test. Rats in 7 groups were raised on diets with different feed calcium and phosphorus concentration for 42 d. Results In the same feed calcium or phosphorus concentration but with different Ca/P ratio, the body weight and bone development of the growing rats were better in the group fed diet with Ca/P ratio of 1.2 ： 1 than that of 0.4：1 and 4：1 （ P 〈 0.05）. With the same feed Ca/P ratio, the body weight and bone development of the growing rats were better in the group fed diet with Ca/P ratio of 1.2： 1, even in the group of LL and HH, but the other groups fed diet with Ca/P ratio of 0.4：1 and 4：1 had significant deviation （ P 〈 0.05）. Conclusion Calcium and phosphorus should be in proportion and in adequate amount in the feed, otherwise the body weight and bone development of the rats may be affected. The results of this study indicate that the body weight and bone development of growing rats are better fed with diet of Ca/P ratio of 1.2：1.