油酸诱导小型猪肺水肿模型的建立

Establishment of Oleic Acid-Induced Animal Model of Pulmonary Edema in Piglets

目的制备油酸诱导小型猪肺水肿的动物模型,便于进行肺水肿的发病机制和相关治疗的研究。方法家养小型猪20只麻醉后随机分A、B两组,A组(n=10)为对照组,B组(n=10)为油酸组(油酸0.15 mL/kg经动物耳缘静脉缓慢注射),观察两组动物肺组织病理改变、计算肺含水量及肺湿干重比。结果B组动物注射油酸后肺部出现明显的肺水肿病理改变,肺湿/干重比及肺含水量的值明显高于A组(P<0.05)。结论本实验成功复制油酸诱导小型猪肺水肿动物模型,其病理组织切片符合肺水肿的典型病变。

Objective To establish an animal model of pulmonary edema in piglets in order to serve the study on mechanisms and treatment of pulmonary edema. Method 20 piglets were randomized into control group （A） and model group （B）, 10 piglets in each group. Oleic acid 0.15 mL/kg was injected slowly into the animals in group B. The pathological changes of the lung tissue were examined, the lung water contents （LWC） and the ratio of lung wet weight （LWW） to lung dry weight （LDW） were calculated. Results The LWC, and the ratio of LWW to LDW in group B was significantly higher than that in group A （ P 〈 0.05 ）. Conclusion An oleic acid-induced animal model of pulmonary edema in piglets has been successfully established. The pulmonary histopathological changes in this model are in accordance with the typical changes of pulmonary edema. It may provide an excellent animal model for studies on mechanisms and treatment of pulmonary edema.