摘要
目的探讨基质金属蛋白酶-2(MMP-2)及张力蛋白和辅助蛋白同源、第10号染色体丢失的磷酸酶(PTEN)的表达在骨肉瘤发生、发展中的作用及其相互关系。方法应用免疫组化SP法检测40例骨肉瘤组织、20例骨软骨瘤组织中MMP-2、PTEN蛋白的表达情况,并分析两者之间表达的相关性。结果MMP-2在骨肉瘤组织中的阳性率为65%(26/40),而在骨软骨瘤组织中的阳性率为0,差异具有显著性(χ2=7.253,P<0.01)。PTEN蛋白在骨肉瘤组织中的阳性表达率为55%(22/40),而在骨软骨瘤组织中为90%(18/20),两者相比差异有统计学意义(χ2=4.164,P<0.05)。在骨肉瘤组织中,MMP-2和PTEN表达强度之间呈负相关(r=-0.403,P<0.05)。结论MMP-2和PTEN与骨肉瘤的发生、发展以及侵袭、转移有关,且两者具有相关性,联合检测MMP-2和PTEN的表达将对骨肉瘤的早期诊断、预后判断有一定指导意义。
Objective To study the expression of matrix mental protein (MMP-2) and (PTEN) in osteosareoma and their clinical significance as well as their correlation Methods Immunohistochemistry (S-P method) was applied to study the expressions of MMP-2 and PTEN in tissues of osteosarcoma (n 40) and osteochondroma (n=20). Results Expression of MMP-2 was positive in 65% of osteosarcoma (26/40) and negative in osteochondroma, while expression of PTEN was found positive in 55% of osteosarcoma (22/40) and 90% in osteochondroma (18/20). The difference between them was significant (χ^2=4. 164,P〈 0.05). Negative correlation was observed between the expression of MMP 2 and PTEN in osteosarcoma (r =-0. 403,P〈0.05). Conclusion MMP-2 and PTEN may be related to the carcinogensis and metastasis of osteosarcoma. Detection of MMP-2 and PTEN expression is of certain significance in early diagnosis and assessment of its prognosis.
出处
《青岛大学医学院学报》
CAS
2007年第6期500-502,共3页
Acta Academiae Medicinae Qingdao Universitatis