大鼠创伤性脑内出血中红细胞对脑水肿的影响

Effects of erythrocyte on brain edema of rat with traumatic intracerebral hemorrhage

目的研究红细胞对创伤性脑内出血(TICH)后脑水肿的影响。方法自由落体打击造成大鼠脑外伤,借助鼠脑立体定向仪向伤区脑皮质内注射全血(WB)、溶解红细胞(LRBC)或压积红细胞(PRBC),制作TICH模型。于伤后1、3、5d处死大鼠,取伤区脑组织测含水量及Na+、K+、Ca2+含量,并作病理检查。结果(1)TBI、WB和PRBC3组第3d的脑含水量最高,LRBC组第1d的含水量最高;4组间比较,第1d时LRBC含水量最高,第3d时PRBC和WB组含水量最高。(2)Na+的变化与含水量的变化相一致。结论TICH所形成的脑水肿较单纯脑外伤重。在TICH中,红细胞对早期脑水肿的形成无明显作用,而对迟发性脑水肿的形成有重要作用。

Objective To study the effects of erythrocyte on brain edema after traumatic intracerebral hemorrhage（TICH）. Methods Firstly, the brain injury model of SD rat was established by applying a free-falling device, then whole blood（WB）,lysed RBC（LRBC） or parked RBC（PRBC） were infused with stereotactic guidance into injury cortex to produce model of TICH. All rats were killed at 1 day, 3days and 5 days after injury. The water content, levels of sodium, potassium and calcium in brain tissue were measured, and pathological changes were observed.Results （1） In group of WB, PRBC and TBI, the brain water content at the third day was the highest. Brain water content of LRBC 1 was markedly higher than that of LRBC 3 and LRBC 5. Comparison among four groups, water content of LRBC was the highest at the 1st day, and that of WB and PRBC were the highest at the 3rd day, and there were no significant differences among that of 4 groups at 5th day. （2） The change of Na＋ coincided with that of water content. Conclusion （1） Brain edema induced by TICH is more significant than that by TBI. （2） Erythrocyte plays an important role in delayed brain edema development （3 days postinjury）, nor does in early stage （1 day after injury）.