神经生长因子在人胚神经管早期发育中的定位表达(英文)

Expression of Nerve Growth Factor during the Development of Neural Tube in Early Human Embryo

目的研究捷安肽素的抗真菌作用机理。方法采用形态学方法和同位素标记法。显微形态观察经捷安肽素处理后的供试真菌的形态学变化。进一步采用14C同位素标记的特异底物"尿苷二磷酸-(14C)-葡萄糖"示踪,研究捷安肽素对真菌(1,3)-β-D-葡聚糖合成酶活性反应的影响。结果研究神经生长因子(NGF)在早期人胚神经管发育过程中的定位表达。方法采用免疫细胞化学ABC法染色,研究35天人胚的发育情况。结果在人胚神经管的室管带中,神经元的细胞质和细胞核NGF免疫反应阳性;在中间带,一部分神经元的细胞核NGF免疫反应阳性,另外一部分神经元的细胞核NGF免疫反应阴性,而其突起NGF免疫反应阳性;在边缘带NGF的表达与中间带相似。在神经管的头侧NGF阳性反应较强,神经管的尾侧NGF阳性反应较弱。结论NGF在人胚神经管免疫反应阳性,表明NGF可能是诱导神经管分化发育的重要信号分子,提示NGF可能在人胚神经管的发育中具有十分重要的作用。

Objective The aim of this study is to investigate the expression and the distribution of the nerve growth factor during the period of neural tube development of human embryo. Method Early development of neural tube was studied in human embryos about 35 gestational days by using immunocytochemical ABC technique. Result There were NGF immuno - positive substances in the cytoplasm and nuclei of neuroepithelial cells in the ventricular zones of neural tube. In the intermediate zone of neural tube, NGF immunoreactivity was detected in the nuclei of some neurons, or the processes of other neurons which contained no NGF - immunoreactive substances in their nuclei; The expression pattern of NGF in the marginal zone of neural tube was similar to that of the intermediate zone. The density of NGF - immunorecative particles was higher on the rostrum side of neural tube than on the caudal side. The NGF immuno - positive cells were also observed among the somites of embryo under the neural tube. Conclusion These results suggest that NGF was an important signal molecule to induce neural tube differentiation, and that NGF may play a significant role in regulation of the biological function of neurons in developing neural tube.